韦 辉1,刘素香2, 3, 4,刘 毅2, 3, 4,张铁军2, 3, 4*,陈常青2*.强肝胶囊HPLC 指纹图谱研究[J].中草药,2013,44():
强肝胶囊HPLC 指纹图谱研究
HPLC fingerprint of Qianggan Capsule
  
DOI:
中文关键词:  关键词:强肝胶囊  HPLC  指纹图谱  化学模式识别  共有模式
英文关键词:Key words: Qianggan Capsule  HPLC  fingerprint  chemical pattern recognition  total mode
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作者单位
韦 辉1,刘素香2, 3, 4,刘 毅2, 3, 4,张铁军2, 3, 4*,陈常青2* 1. 天津中医药大学天津 300193 2. 天津药物研究院天津 300193 3. 天津市中药质量控制技术工程实验室天津 300193 4. 中药现代制剂与质量控制技术国家地方联合工程实验室(天津)天津 300193 
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中文摘要:
      摘 要:目的 建立强肝胶囊HPLC 指纹图谱并对其进行化学模式识别。方法 采用Diamonsil C18 柱(250 mm×4.6 mm,5 μm),在230 nm 波长下,以乙腈-0.05%磷酸水溶液进行梯度洗脱,测定了44 批强肝胶囊样品;应用相似度分析软件建立强 肝胶囊指纹图谱的共有模式,并对色谱峰进行了指认。结果 建立强肝胶囊HPLC 指纹图谱,在确定的方法下,得到44 批 强肝胶囊的色谱图,并获得了44 批样品的相似度;确定共有峰14 个,其中13 个归属到各药材,11 个已确认成分。结论 强 肝胶囊的HPLC 指纹图谱的构建和共有色谱峰成分的确定为强肝胶囊药品质量控制提供更全面的参考。
英文摘要:
      Abstract: Objective To develop a new method for the isolation and purification of cordycepin by high speed counter-current chromatography (HSCCC) with Cordyceps militaris fermentation broth as raw material. Methods The solvent systems for cordycepin separation were assessed and selected by HPLC partition coefficient method and analytical HSCCC. A solvent system that consisted of ethyl acetate-n-butanol-0.5% ammonia water (2:3:5) was applied to the separation. The upper phase was used as the stationary phase, while the lower phase was used as the mobile phase for the cordycepin separation by HSCCC. Results A high efficiency of HSCCC separation was achieved. Finally, cordycepin (43.8 mg) was obtained from 400 mg crude extract of C. militaris fermentation broth in one-step separation with a purity of 98.7%. Conclusion HSCCC is an efficient and simple method for the largescale preparation of cordycepin from C. militaris fermentation broth, which could provide the material basis for the pharmacological research of cordycepin .
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