高 飞1,傅超美1,胡慧玲1*,王战国2,傅 舒1,毛 茜1,盛菲亚1.HPLC-UV 技术用于川木香煨制前后代谢物轮廓差异研究[J].中草药,2013,44():
HPLC-UV 技术用于川木香煨制前后代谢物轮廓差异研究
Study on the differentiates of metabolic profiling of Vladimiriae Radix before and after processing using HPLC-UV
  
DOI:
中文关键词:  关键词:HPLC-UV  代谢组学  代谢物轮廓  川木香  煨制
英文关键词:Key words: HPLC-UV  metabonomics  metabolic profiling  Vladimiriae Radix  processed
基金项目:基金项目:国家自然科学基金青年基金项目(81001638)
作者单位
高 飞1,傅超美1,胡慧玲1*,王战国2,傅 舒1,毛 茜1,盛菲亚1 1. 成都中医药大学药学院 中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地四川 成都 611137 2. 四川大学生命科学院四川 成都 610041 
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中文摘要:
      摘 要:目的 比较川木香煨制前后代谢物轮廓(metabolic profiling)差异,筛选出导致差异的特征性化学成分。方法 经HPLC-UV 分析获得川木香生品、煨品的色谱图数据集;采用主成分分析(principal component analysis,PCA)、偏最小二乘法-判别分析(partial least square-discriminant analysis,PLS-DA)、聚类分析(hierarchical cluster analysis,HCA)等多变量统计分析方法比较川木香生品、煨品的代谢物轮廓差异及筛选导致差异的特征性化学成分。结果 川木香生品和煨品代谢物轮廓明显不同,导致差异的特征性化学成分有7 个(包含木香烃内酯和去氢木香内酯)。结论 从代谢物轮廓角度,川木香煨制前后具有显著差异。
英文摘要:
      Abstract: Objective Comparing the metabolic profiling differences of Vladimiriae Radix before and after processing, screen out the discrepant constituents. Methods Acquiring the chromatogram information from the processed and unprocessed Vladimiriae Radix by UV-HPLC analysis. Then comparing the metabolic profiling differences of Vladimiriae Radix before and after processing by PCA, PLS-DA, HCA, and screen out the discrepant constituents which lead up to these differences. Results The metabolic profilings between processed and unprocessed Vladimiriae Radix are apparent different, data showed 7 peaks of them mainly make the quality difference, include active constituents costunolide and dehydrocostus lactone. Conclusion Based on the metabolic profiling can be used to study the processing mechanism of Vladimiriae Radix.
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