毕海燕,刘静,马莉,吕雄文,张铁军.疏风解毒胶囊对肺炎大鼠抗炎作用的配伍合理性研究[J].中草药,2019,(15):3541-3546
疏风解毒胶囊对肺炎大鼠抗炎作用的配伍合理性研究
Study on rationality of anti-inflammatory effect of Shufeng Jiedu Capsule on pneumonia rats
投稿时间:2019-03-05  
DOI:10.7501/j.issn.0253-2670.2019.15.004
中文关键词:  疏风解毒胶囊  抗炎作用  细菌计数  白细胞  嗜酸性粒细胞  嗜碱性粒细胞  核转录因子-κB  环加氧酶2
英文关键词:Shufeng Jiedu Capsule  anti-inflammatory effect  bacterial count  leukocyte  eosinophil  basophil  NF-κB  COX-2
基金项目:国家中药标准化专项资助(ZYBZH-C-AH-02);国家自然科学基金资助项目(81430096);国家自然科学基金资助项目(81703802)
作者单位E-mail
毕海燕 安徽医科大学药学院, 安徽合肥 230032  
刘静 天津药物研究院新药评价有限公司, 天津 300301  
马莉 天津药物研究院新药评价有限公司, 天津 300301  
吕雄文 安徽医科大学药学院, 安徽合肥 230032 lxw31288@aliyun.com 
张铁军 天津药物研究院, 中药现代制剂与质量控制技术国家地方联合工程实验室, 天津 300193
天津药物研究院, 天津市中药质量标志物重点实验室, 天津 300193 
zhangtj@tjipr.com 
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中文摘要:
      目的 以疏风解毒胶囊全方及其中清热解毒、解表组分为研究对象,考察疏风解毒胶囊组方配伍对肺炎模型大鼠的抗炎作用。方法 制备肺炎链球菌致肺炎大鼠模型,分为对照组、模型组、头孢氨苄组、疏风解毒胶囊全方组(全方组)、疏风解毒胶囊解表组(解表组)、疏风解毒胶囊清热解毒组(清热解毒组),连续给药6 d,检测各组大鼠全血及肺泡冲洗液细菌计数、全血及肺泡冲洗液白细胞(WBC)计数及分类以及血清核转录因子-κB(NF-κB)、环加氧酶-1(COX-1)、COX-2水平,评价组方配伍的合理性。结果 与模型组比较,疏风解毒胶囊全方组、解表组、清热解毒组大鼠外周血与肺泡冲洗液菌落计数显著降低,外周血WBC数量显著降低,嗜酸性粒细胞比例显著升高,嗜碱性粒细胞比例显著降低;全方组大鼠在WBC数量及嗜酸性粒细胞比例2个指标上Q值>1;全方组、解表组、清热解毒组大鼠肺泡冲洗液WBC数量显著减少,血清中NF-κB、COX-2水平显著降低。结论 疏风解毒胶囊对肺炎链球菌感染的大鼠有显著的治疗作用,解表组分和清热解毒组分有显著的协同作用。
英文摘要:
      Objective In order to investigate the effect of Shufeng Jiedu Capsule (SJC) compatibility on anti-inflammation of pneumonia model rats. SJC whole prescription and its components with effect of heat-clearing and toxin-removing and exterior-releasing were studied. Methods The pneumonia model of rats induced by Streptococcus pneumoniae was used, divided into control group, model group, cephalexin group, SJC whole prescription group (whole prescription group), SJC exterior-releasing group (exterior-releasing group), and SJC heat-clearing and toxin-removing group (heat clearing and detoxifying group). After administration for 6 d, the colony count of peripheral blood and alveolar lavage fluid (BALF), the number of white blood cell (WBC) and its classification in peripheral blood and alveolar lavage fluid, the contents of nuclear transcription factor-kappa B (NF-κB) and cyclooxygenase (COX-1 and COX-2) were tested to evaluate the compatibility rationality. Results Compared with model group, the whole prescription group, exterior-releasing group, and heat-clearing and toxin-removing group significantly reduced the colony count of peripheral blood and alveolar lavage fluid, significantly reduced the number of WBC in peripheral blood, significantly increased the proportion of eosinophils and reduced the proportion of basophils. The Q values of rats in the whole prescription group were beyond 1 on two indicators, which were the number of WBC and the proportion of eosinophils. The number of WBC in alveolar lavage fluid and the contents of NF-κB and COX-2 decreased significantly in the whole prescription group, exterior-releasing group and heat-clearing and toxin-removing group. Conclusion SJC has significant anti-inflammatory effect and therapeutic effect on Streptococcus pneumoniae infection in rats. The exterior-relieving component and the heat-clearing and toxin-removing component have significant synergistic effects.
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