邰 文,刘东博,吴 疆,潘明佳,陈常青.T细胞受体基因修饰在过继免疫治疗中的应用[J].药物评价研究,2011,34(2):105-109
T细胞受体基因修饰在过继免疫治疗中的应用
Application of T cell receptor gene modification in adoptive immunotherapy
  
DOI:10.7501/j.issn.0253-6376.[year].[issue].[sequence]
中文关键词:  T细胞;基因修饰;T细胞受体;基因转移;过继免疫治疗
英文关键词:T cell; gene modification; T cell receptor; gene transfer; adoptive immunotherapy
基金项目:
作者单位
邰 文,刘东博,吴 疆,潘明佳,陈常青 天津药物研究院,天津 300193 
摘要点击次数: 3163
全文下载次数: 5832
中文摘要:
      T细胞过继性转移可以增强免疫系统介导的对肿瘤细胞的消除,是近年来获得较高关注的一种特异性、无毒性的癌症新疗法,此方法对于治疗血液性和实体恶性肿瘤具有一定效果。对T细胞进行基因修饰能够增强其免疫能力,保持T细胞的持久活性,同时也可以克服肿瘤自身的免疫逃避机制,潜在提高免疫治疗应用于多种肿瘤疾病的成功率。在T细胞过继转移中,T细胞受体(TCR)基因转移作为一种发展迅速的免疫治疗方法,可以在体外产生大量的具有已知抗原特异性和功能亲和性的T细胞,应用于病毒感染或病毒相关恶性肿瘤的过继细胞免疫治疗。TCR基因转移利用逆转录病毒或慢病毒作为载体,其中包含了从所需抗体特异性T细胞群中克隆得到的TCR-α和TCR-β链基因序列,然后应用TCR编码载体转导体外的原始T细胞。为产生带有所需功能特异性的转导T细胞,引入的TCR-α和TCR-β链必须形成异源二聚体,与CD3复合体结合从而在T细胞表面稳定表达。
英文摘要:
      T cell adoptive transfer could enhance immune-mediated elimination of tumor cells with high concerns for providing a specific and non-toxic cancer therapy,. This approach has been effective in treating some hematologic and solid malignancies. Moreover, genetic modification of T cells could improve their immunopotency, maintain their persistence, and overcome the tumor immune evasion mechanisms as well, so as to potentially increase the achievement rate of immunotherapy in a wide range of tumors. In T cell adoptive transfer, T cell receptor (TCR) gene transfer, a rapid developing strategy of immunotherapy, can generate a number of T cells with a given antigen-specificity and functional avidity in vitro and is used to treat viral infectious diseases and virus-associated malignancies and caner. TCR gene transfer utilizes retrovirus or lentivirus as vectors which contain gene sequences of the TCR-α and β chains. TCR-α and -β chains were cloned from a T-cell population with the desired antigen specificity. Then the TCR encoding vector is used to transduce primary T cells in vitro. To generate a transduced T cell with the disired functional specificity, the introduced TCR-α and -β chains must form a heterodimer and combine with the CD3 complex in order to be stably expressed at the T-cell surface.
HTML  查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 2337948 位访客
版权所有 天津中草药杂志社 (天津市南开区鞍山西道308号 300193)
Address: 308# An-shan West Road, Nankai District, Tianjin 300193, China
Tel: +86-22-27474913; 23006822 Fax: +86-022-23006822 E-mail:zcy@tiprpress.com
津备案:津ICP备13000267号 互联网药品信息服务资格证书编号:(津)-非经营性-2015-0031