陈玉,张可佳,李孝庆,宋兆辉,余伯阳,鄂秀辉.胡黄连中环烯醚萜苷类成分体外抗非酒精性脂肪性肝炎活性研究[J].药物评价研究,2019,42(5):846-851
胡黄连中环烯醚萜苷类成分体外抗非酒精性脂肪性肝炎活性研究
Anti-NASH activity of Iridoid Glycosides in Picrorhizae Rhizoma in vitro
投稿时间:2019-01-18  
DOI:10.7501/j.issn.1674-6376.2019.05.007
中文关键词:  非酒精性脂肪性肝炎;胡黄连苷Ⅰ;胡黄连苷Ⅱ;胡黄连苷Ⅲ;胡黄连苷Ⅳ;氧化应激;炎症
英文关键词:NASH;PicrosideⅠ;PicrosideⅡ;PicrosideⅢ;PicrosideⅣ;cell model
基金项目:
作者单位E-mail
陈玉 中国药科大学 江苏省中药评价与转化重点实验室, 江苏 南京 210009
天士力控股集团有限公司研究院 现代中药开发中心, 天津 300410
天士力制药集团股份有限公司 创新中药关键技术国家重点实验室, 天津 300410 
 
张可佳 天士力控股集团有限公司研究院 现代中药开发中心, 天津 300410
天士力制药集团股份有限公司 创新中药关键技术国家重点实验室, 天津 300410 
 
李孝庆 天士力控股集团有限公司研究院 药理毒理开发中心, 天津 300410  
宋兆辉 天士力控股集团有限公司研究院 现代中药开发中心, 天津 300410
天士力制药集团股份有限公司 创新中药关键技术国家重点实验室, 天津 300410 
 
余伯阳 中国药科大学 江苏省中药评价与转化重点实验室, 江苏 南京 210009 boyangyucpu@163.com 
鄂秀辉 天士力控股集团有限公司研究院 现代中药开发中心, 天津 300410
天士力制药集团股份有限公司 创新中药关键技术国家重点实验室, 天津 300410 
exh@tasly.com 
摘要点击次数: 25
全文下载次数: 43
中文摘要:
      目的 探究胡黄连苷Ⅰ、Ⅱ、Ⅲ、Ⅳ体外抗非酒精性脂肪性肝炎(NASH)的活性。方法 用1 mmol/L游离脂肪酸(FFA)诱导HepG2细胞24 h,建立体外NASH模型;MTT法检测胡黄连苷Ⅰ、Ⅱ、Ⅲ、Ⅳ对细胞活力的影响,并依此优选3个安全给药浓度(0.25、0.50、1.00 mg/mL)干预体外NASH模型;油红O染色法镜下观察细胞内脂滴的变化情况;试剂盒法检测细胞培养上清液中超氧化物歧化酶(SOD)、丙二醛(MDA)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)的含量变化。结果 胡黄连苷Ⅰ、Ⅱ、Ⅲ、Ⅳ对HepG2细胞的活力影响较小。FFA刺激24 h后,模型组与对照组比较,细胞内被染成明显的红色颗粒状脂滴,细胞培养上清液中SOD水平显著下降,MDA、TNF-α、IL-8水平显著上升,均有显著性差异(P<0.05、0.01)。与模型组比较,不同浓度的胡黄连苷Ⅰ、Ⅱ、Ⅲ、Ⅳ干预组,细胞内红色颗粒状脂滴减少,且以胡黄连苷Ⅱ最为明显;SOD水平显著上升,MDA、TNF-α、IL-8水平显著下降(P<0.05、0.01),且以胡黄连苷Ⅱ、Ⅲ、Ⅳ较为明显,但没有显著的剂量相关性。结论 胡黄连苷Ⅰ、Ⅱ、Ⅲ、Ⅳ中胡黄连苷Ⅱ的体外抗NASH的活性较显著。
英文摘要:
      Objective To discuss the anti-NASH activity of PicrosideⅠ, PicrosideⅡ, PicrosideⅢ and PicrosideⅣ in vitro.Methods Liver cells HepG2 were cultured and induced by FFA to establish NASH cell model. The cell viability were measured by MTT to select the safe drug concentration. Then safe concentrations of PicrosideⅠ, PicrosideⅡ, PicrosideⅢ and PicrosideⅣ were meanwhile added. The fat deposition in cells was observed by oil-red O staining method. The content of SOD, MDA, IL-8 and TNF-α were tested by Elisa. Results Three treatment groups were seted with the concentration of 0.25, 0.50 and 1.00 mg/mL. Compared with control group, the NASH cell model group was stained with obvious red granular lipid droplets. The levels of SOD were significantly higher in model group than the control group, while MDA, TNF-α and IL-8 significantly lower than control group (P<0.05 and 0.01). Compared with the model group, the red granular lipid droplets decreased in the intervention groups of different concentrations of Picroside I, Ⅱ, Ⅲ and IV, especially Picroside Ⅱ. SOD levels increased significantly, MDA, TNF-a and IL-8 levels decreased significantly (P<0.05, 0.01), and Picroside Ⅱ, Ⅲ and IV were more obvious, but there was no significant dose correlation. Conclusion PicrosideⅡcan improve cellular pathology and influence the levels of SOD, MDA, IL-8 and TNF-α obviously, showing potential value on NASH treatment.
HTML  查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 2162102 位访客
版权所有 天津中草药杂志社 (天津市南开区鞍山西道308号 300193)
Address: 308# An-shan West Road, Nankai District, Tianjin 300193, China
Tel: +86-22-27474913; 23006822 Fax: +86-022-23006822 E-mail:zcy@tiprpress.com
津备案:津ICP备13000267号 互联网药品信息服务资格证书编号:(津)-非经营性-2015-0031