李 媛1, 2,许红涛1, 2,李华强3,蒙雄裕4,王 亮1,张 宏1*,秦路平1*.肾阳虚骨质疏松大鼠动物模型的建立[J].药物评价研究,2015,38():1-5
肾阳虚骨质疏松大鼠动物模型的建立
Establishment of animal model of osteoporosis with kidney yang deficiency syndrome in rats
投稿时间:2014-12-26  
DOI:
中文关键词:  肾阳虚;骨质疏松;去势;氢化可的松
英文关键词:kidney yang deficiency; osteoporosis; ovariectomy; hydrocortisoneHydrocortisone
基金项目:国家科技部863计划:抗骨质疏松海洋中药制剂-资骨胶囊的研究(2013AA093003)
作者单位
李 媛1, 2,许红涛1, 2,李华强3,蒙雄裕4,王 亮1,张 宏1*,秦路平1* 1. 上海第二军医大学药学院生药教研室,上海 200433
2. 福建中医药大学药学院,福建 福州 350108
3. 上海师范大学生命与环境科学学院,上海 200234
4. 宁夏医科大学药学院,宁夏 银川 750004 
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中文摘要:
      目的 建立肾阳虚骨质疏松大鼠病证结合动物模型。方法 大鼠按体质量随机分为3组,分别为实验组16只、去卵巢组16只及假手术组16只。实验组大鼠采用双侧去卵巢并im氢化可的松的方法进行造模。造模期间对大鼠一般情况进行观察及对生理状态量化检测。3个月后,采用ELISA法检测大鼠血清环磷酸腺苷(cAMP)、环磷酸鸟苷(cGMP)、雌二醇(E2)、睾酮(T)、促肾上腺皮质激素(ACTH)、三碘甲状腺原氨酸(T3)、四碘甲状腺原氨酸(T4)水平。用电子天平称肾上腺,甲状腺及胸腺湿质量,并取股骨进行骨密度和骨矿含量的检测。结果 给药造模2周后,实验组大鼠出现毛发干枯、畏寒喜暖、卷缩弓背、小便清长等症状,体质量增长明显迟缓,血清cAMP水平较假手术组显著下降。3个月后,实验组大鼠自发活动次数明显减少,血清cAMP、E2、ACTH、T3、T4水平明显下降,骨密度、骨矿含量显著降低,肾上腺及甲状腺系数明显低于假手术组。结论 去势并im氢化可的松建立肾阳虚骨质疏松大鼠动物模型是一种可行的方法。
英文摘要:
      Objective To establish an animal model of osteoporosis with kidney yang deficiency syndrome in rats. Methods Rats were randomly divided into three groups by body weight, They were experimental, ovariectomized, and Sham operation groups (16 in each group). In the experimental group, rats were subjected to bilateral ovarian resection followed by im injection of hydrocortisone. The general conditions and physiological conditions were observed and measured during the period of the experiment. After three months, the serum levels of cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP), estrogen (E2), testosterone (T), adrenocorticotropic hormone (ACTH), three typical thyroid original acid (T3), and four typical thyroid original acid (T4) were determined by ELISA method. The fresh weights of adrenal, thymus, and thyroid were collected by using electronic balance. In addition, the bone mineral density (BMD) and bone mineral content (BMC) were detected. Results Two weeks after hydrocortisone treatment, it was observed that the rats in the experimental group displayed kidney yang deficiency syndrome such as dry hairs, chills, back arched, and clear abundant urine. Correspondingly, the body weight increased slowly. The level of cAMP was significantly lower than that of rats in the Sham operation group. Three months later, spontaneous activities of rats in the experimental group markedly decreased, the content of cAMP, E2, ACTH, T3, and T4 significantly decreased when compared with the rats in the Sham operation group. The BMD and BMC decreased obviously, showing a notable statistic difference between the experimental groupand Sham operation group. The coefficient of adrenal and thyroid was lower than that of rats in the Sham operation group. Conclusion Bilateral ovarian resection followed by im injection of hydrocortisone may be a credible method for establishing the animal model of osteoporosis with kidney yang deficiency syndrome.
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