陆 洁,刘 静,裴天仙,田兴美,申秀萍*.自发性2型糖尿病模型db/db小鼠生物学特性研究[J].药物评价研究,2013,36():1-5
自发性2型糖尿病模型db/db小鼠生物学特性研究
Biological characteristics of spontaneous Type 2 diabetic db/db mice
投稿时间:2013-03-22  
DOI:
中文关键词:  db/db小鼠;2型糖尿病;生物学特性;免疫组化双重染色;胰岛素抵抗
英文关键词:db/db mice; type 2 diabetes; biological characteristics; immunohistochemical double staining; insulin resistance
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作者单位
陆 洁,刘 静,裴天仙,田兴美,申秀萍* 天津药物研究院 天津市新药安全评价研究中心,天津 300193 
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中文摘要:
      目的 对db/db小鼠进行生物学特性研究,满足糖尿病研究的需要。方法 在试验周期内,测定db/db小鼠的空腹血糖、体质量及摄食饮水量,并测定21周龄模型动物的糖耐量、血中胰岛素、胰高血糖素、三酰甘油、总胆固醇等指标的水平,进行胰腺的免疫组化双重染色及主要脏器的病理学检查,并与db/m小鼠进行比较。结果 与对照组db/m小鼠比较,db/db小鼠过度肥胖,伴有高血糖、胰岛素抵抗、脂质代谢紊乱,且肝脏和胰腺组织均出现明显病变,胰腺免疫组化双重染色结果与血液学测定结果一致,并可观察到胰岛中A细胞和B细胞的分布变化情况。结论 对自发性2型糖尿病模型db/db小鼠所做的相关生物学特性检测,可供糖尿病研究参考。
英文摘要:
      To investigate the biological characteristics of db/db mice to meet the need of study on diabetes mellitus. Methods In this experiment, the fasting blood glucose, body weight, and food and water intake were measured. The carbohydrate tolerance, insulin, glucagon, triglyceride, and total cholesterol in serum were tested on db/db mice of 21 weeks. Meanwhile, the immunohistochemical double staining in pancreas and morphological study was carried out. All the data were compared with db/m mice in statistics. Results Compared with db/m mice, obesity, hyperinsulinemia, hyperglycemia, and lipid metabolism disorder were serious on db/db mice, following by obvious pathological deterioration of liver and pancreas. According to the result of blood testing, the changes in the distribution of A cells and B cells could be observed in immunohistochemical double staining on pancreas of db/db mice. Conclusion The db/db mice could be used as appropriate disease model of type 2 diabetes mellitus, providing relevant biological information for further study in this field.
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