杨静,高纳影,赵艳敏,李覃,刘岱琳.固公果根提取物促进结肠癌细胞凋亡的机制研究[J].药物评价研究,2019,42(9):1757-1762
固公果根提取物促进结肠癌细胞凋亡的机制研究
Mechanisms of extracts of roots of Rose odorata sweet var. gigantean on colon cancer cells via promoting apoptosis
投稿时间:2019-03-04  
DOI:10.7501/j.issn.1674-6376.2019.09.008
中文关键词:  结肠癌细胞;固公果根提取物;细胞凋亡;凋亡蛋白;氧化应激
英文关键词:colon cancer cells;extract of roots of Rose odorata sweet var. gigantean (ROE);apoptosis;apoptotic protein;oxidative stress
基金项目:国家自然科学基金资助项目(81673693)
作者单位E-mail
杨静 天津中医药大学, 天津 301617
中国人民武装警察部队后勤学院 卫生勤务系军事药学教研室, 天津 300309 
 
高纳影 中国人民武装警察部队后勤学院 卫生勤务系军事药学教研室, 天津 300309  
赵艳敏 天津中医药大学, 天津 301617
中国人民武装警察部队后勤学院 卫生勤务系军事药学教研室, 天津 300309 
 
李覃 中国人民武装警察部队后勤学院 卫生勤务系军事药学教研室, 天津 300309  
刘岱琳 中国人民武装警察部队后勤学院 卫生勤务系军事药学教研室, 天津 300309 dailinlch@163.com 
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中文摘要:
      目的 探讨固公果根提取物促进结肠癌细胞凋亡及机制研究。方法 分别采用MTT法、DAPI染色及流式细胞术检测固公果根提取物对结肠癌细胞增殖活性及细胞凋亡的影响;蛋白免疫印迹(Western-Blot)法检测凋亡相关蛋白的活性表达水平。结果 固公果根提取物能显著抑制结肠癌SW620和LOVO细胞增殖,促进两种结肠癌细胞凋亡,上调凋亡相关基因Keap-1、Bax的蛋白表达,下调Nrf2、HO-1以及Bcl2蛋白的表达。结论 固公果根提取物能显著抑制人结肠癌细胞SW620和LOVO增殖、诱导细胞凋亡,其作用机制可能涉及调控凋亡相关基因Bax、Bcl-2表达,且与激活Nrf2氧化应激通路有关。
英文摘要:
      Objective To investigate effects and mechanism of the extract of roots of Rose odorata sweet var. gigantean (ROE) on apoptosis of colon cancer cells. Methods MTT assay, DAPI staining, and flow cytometry were used to detect the effects of ROE extracts on proliferation and apoptosis of colon cancer cells. The protein expression was detected by Western Blotting. Results ROE extracts could significantly inhibit the growth of SW620 and LOVO cells and promote it apoptosis. ROE extracts could also up-regulate the expression of Keap-1 and Bax protein, and down-regulate the expression of Nrf2, HO-1 and Bcl2 protein. Conclusion ROE extracts can significantly inhibit the proliferation and induce apoptosis of human colon cancer cells SW620 and LOVO, which mechanisms might be involved in regulating the expression of apoptosis-related genes Bax and Bcl-2 and activating Nrf2 oxidative stress pathway.
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