杨莉,王昭昕,卢国彦,王跃飞,张玥,周昆.补骨脂不同提取部位大鼠给药3个月的毒性比较研究[J].药物评价研究,2019,42(8):1531-1536
补骨脂不同提取部位大鼠给药3个月的毒性比较研究
Comparative study on three months toxicity of rats administration with different parts extracts of Psoralea corylifolia
投稿时间:2019-01-14  
DOI:10.7501/j.issn.1674-6376.2019.08.008
中文关键词:  补骨脂;提取物;毒性试验
英文关键词:Psoralea corylifolia;extract;toxicity test
基金项目:国家自然科学基金(81673826,81703790);国家重大新药创制专项(2014ZX09304307-001-005)
作者单位E-mail
杨莉 天津中医药大学 中医药研究院, 天津 300193  
王昭昕 天津中医药大学 中医药研究院, 天津 300193  
卢国彦 天津中医药大学 中医药研究院, 天津 300193  
王跃飞 天津中医药大学 中医药研究院, 天津 300193  
张玥 天津中医药大学 中医药研究院, 天津 300193
天津中医药大学 方剂学教育部重点实验室, 天津 300193 
zhangyue@tjutcm.edu.cn 
周昆 天津中医药大学 中医药研究院, 天津 300193
天津中医药大学 方剂学教育部重点实验室, 天津 300193 
 
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中文摘要:
      目的 研究补骨脂不同提取部位大鼠长期ig给药的毒性反应。方法 按照一定的方法制备得到补骨脂不同部位提取物,即渗漉药渣、上层渗漉液和混合物。将SD大鼠98只,按体质量、性别随机分为7组,即对照组,补骨脂药渣高、低剂量组,补骨脂干膏高、低剂量组,补骨脂混合物高、低剂量组(高、低剂量均相当于生药6、3 g/kg),每组14只。以1 mL/100 g剂量ig给药12周。每周称大鼠体质量,给药结束后,剖取脏器,计算脏器系数;取血,检测血清生化指标;取肝脏和肾脏组织进行组织病理学检查。结果 连续给药12周后,与对照组相比,混合物高剂量组以及干膏高剂量组雄性大鼠体质量显著降低(P<0.05、0.01);与对照组相比,随给药时间的延长,干膏低剂量组雌性大鼠体质量显著降低(P<0.05),其余给药组无显著差异。除干膏高、低剂量组外,其余各组雄性、雌性大鼠肝脏系数均显著高于对照组(P<0.01、0.001)。与对照组比较,雄性大鼠混合物高剂量组以及药渣高、低剂量组的丙氨酸氨基转换酶(ALT)、三酰甘油(TG)显著下降(P<0.05、0.01),混合高剂量组和干膏高、低剂量组尿素氨(BUN)显著降低(P<0.05、0.01、0.001)。与对照组相比,雌性大鼠混合物高剂量组和干膏高剂量组总胆汁酸(TBA)显著升高(P<0.01),混合物高剂量组和药渣高、低剂量组总胆红素(TBIL)显著升高(P<0.05、0.01)。结论 大鼠长期ig给予补骨脂不同提取物可造成一定的毒性反应,且具有一定的剂量相关性,主要表现为肝脏和肾脏功能损害,并且损伤程度存在雌雄差异。
英文摘要:
      Objective To study the toxicity of different extracts of Psoralea corylifolia by long-term ig administration in rats. Methods The extracts from different parts of Psoralea corylifolia were prepared by a certain method, namely, osmotic residue, superficial osmotic solution, and mixture. 98 SD rats were randomly divided into 7 groups according to body weight and sex, namely, control group, high and low dose group of Psoralea corylifolia residue, high and low dose group of Psoralea corylifolia dry ointment, and high and low dose group of Psoralea corylifolia mixture (high and low doses were equivalent to 6, 3 g/kg of crude drugs), and each group had 14 rats. 1 mL/100 g was ig administered for 12 weeks. The weight of the rats were weighed every week. After treatment, the organs were dissected and the organ coefficients were calculated, the serum biochemical indicators was separately measured. And liver and kidney tissues were taken for histopathological examination. Results After 12 weeks of continuous administration, compared with control group, the body weight of male rats in the high-dose group of mixture and highdose group of dry ointment decreased significantly (P<0.05, 0.01), compared with the control group, the body weight of female rats in the low-dose group of dry ointment decreased significantly with the prolongation of administration time (P<0.05), while there was no significant difference in the other groups. In addition to the high and low dose groups of dry ointment, the liver coefficients of the female and male rats in the other extract groups were significantly higher than those in the control group (P<0.01, 0.001). Compared with the control group, the ALT and TG of male rats were significantly decreased in the high-dose group of mixture, and the high and low dose group of residue (P<0.05, 0.01), and the BUN were significantly decreased in the high-dose group of mixture and the high and low dose groups of dry ointment (P<0.05, 0.01, 0.001). Compared with the control group, TBA of female rats was increased significantly in the high-dose group of mixture and the high-dose group of dry ointment (P<0.01), TBRL was increased significantly in the high-dose group of mixture and the high and low-dose group of residue (P<0.05, 0.01).Conclusion Long-term ig administration of different extracts of Psoralea corylifolia can cause certain toxicity in rats, and it has a dose-dependent effect. The main manifestations are liver and kidney function damage, and the degree of damage is different between male and female.
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