朱世杰,张明阳,秦源,刘兆昌,李潺,田维毅,唐中生,罗亚非,陆莹,范瑞娟.黄芪多糖对蛛网膜下腔出血大鼠神经元凋亡因子及脑积水的影响[J].药物评价研究,2019,42(8):1509-1514
黄芪多糖对蛛网膜下腔出血大鼠神经元凋亡因子及脑积水的影响
Effects of astragalus polysaccharide on neuronal apoptotic factor and hydrocephalus in SAH rats
投稿时间:2019-01-23  
DOI:10.7501/j.issn.1674-6376.2019.08.004
中文关键词:  黄芪多糖;蛛网膜下腔出血;凋亡;脑积水
英文关键词:APS;SAH;apoptosis;hydrocephalus
基金项目:贵州省国内一流建设学科项目(中药学GNYL[2017]008号);贵中医科院内[2016]30号
作者单位E-mail
朱世杰 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
张明阳 辽宁师范大学, 辽宁 大连 116029  
秦源 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
刘兆昌 大连医科大学 基础医学院, 辽宁 大连 116044  
李潺 大连医科大学 基础医学院, 辽宁 大连 116044  
田维毅 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
唐中生 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
罗亚非 贵州中医药大学 基础医学院, 贵州 贵阳 550025 944943527@qq.com 
陆莹 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
范瑞娟 贵州中医药大学 基础医学院, 贵州 贵阳 550025  
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中文摘要:
      目的 通过研究黄芪多糖对蛛网膜下腔出血(SAH)大鼠神经元凋亡因子及脑积水的影响,探讨黄芪多糖发挥作用可能存在的机制。方法 以雄性SD大鼠为研究对象,随机分为假手术组、模型组、黄芪多糖组。采用颅内血管刺破法建立SAH模型后,黄芪多糖组ip给予黄芪多糖40 mg/kg,1次/d,模型组给予和假手术组相同容量的生理盐水,24 h后进行神经功能评分,采用免疫组化和免疫蛋白印迹法检测各组大鼠海马部核因子(NF)-κB、Bcl2、Caspase3、AQP4的阳性细胞数及灰度值,应用核磁共振技术分别扫描各组大鼠脑室,统计脑室大小指数变化情况。结果 免疫组化和免疫蛋白印迹结果显示,与假手术组相比,黄芪多糖组和模型组大鼠海马部凋亡相关因子Bcl2、Caspase3、NF-κB均有明显的表达(P<0.01),但模型组上调Bcl2的表达和抑制Caspase3及NF-κB表达的效果弱于黄芪多糖组(P<0.01)。与假手术组对比,黄芪多糖组和模型组海马部AQP4的表达和脑室指数均明显提高(P<0.01),黄芪多糖组与模型组相比海马部AQP4表达无统计学意义,而黄芪多糖组脑室指数小于模型组(P<0.01)。结论 黄芪多糖能通过调节NF-κB、Bcl2、Caspase3的表达来抑制SAH大鼠海马部位神经元的凋亡,而减轻因SAH引起的脑积水,在早期可能不是主要通过调节AQP4表达而发挥作用的。
英文摘要:
      Objective To study the effect of astragalus polysaccharide on neuronal apoptosis factor and hydrocephalus in rats with subarachnoid hemorrhage and explore the possible mechanism of astragalus polysaccharide.Methods Male SD rats were randomly divided into sham operation group, model group and astragalus polysaccharide group. After the SAH model was established by intracranial vascular puncture, the astragalus polysaccharide group was intraperitoneally injected with astragalus polysaccharide (40 mg/kg, once a day). The model group was given the same volume of saline as the sham-operated group. After 24 hours, the neurological function was scored. The positive cells of NF-κB, Bcl 2, Caspase 3 and AQP4 in the hippocampus of each group were detected by immunohistochemistry and Western blotting. The ventricles of rats in each group were scanned by magnetic resonance technology, and the changes of ventricular size index were counted.Results The results of immunohistochemistry and Western blot showed that the expression of Bcl 2, caspase 3 and NF-κB in the hippocampus of ASP group and model group were significantly higher than those of sham operation group (P<0.01), but the effect of up-regulating the expression of Bcl 2 and down-regulating the expression of caspase 3 and NF-κB in model group was weaker than that of ASP group (P<0.01). Compared with the sham operation group, the expression of AQP4 in hippocampus and ventricular index in Astragalus polysaccharide group and model group were significantly increased (P<0.01). The expression of AQP4 in hippocampus of astragalus polysaccharide group was not statistically significant, but the ventricular index in astragalus polysaccharide group was lower than that in model group (P<0.01). Conclusion Astragalus polysaccharide can inhibit neuronal apoptosis in the hippocampus of SAH rats by regulating the expression of NF-κB, Bcl 2 and caspase 3, and alleviate hydrocephalus caused by SAH. It may not play a major role in the early stage by regulating the expression of AQP4.
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