耿 越,赵 红.马尾松花粉硫酸酯化多糖对大鼠主动脉平滑肌细胞 [Ca2+]i调控及增殖的影响[J].药物评价研究,2014,37():
马尾松花粉硫酸酯化多糖对大鼠主动脉平滑肌细胞 [Ca2+]i调控及增殖的影响
Effect of sulfated polysaccharide from masson pine pollen on [Ca2+]i regulation and proliferation of arterial smooth muscle cells from rats
  
DOI:
中文关键词:  松花粉;硫酸酯化多糖;动脉平滑肌细胞;钙离子;增殖
英文关键词:masson pine pollen; sulfated polysaccharide; arterial smooth muscle cells; calcium ion; proliferation
基金项目:山东省自然科学基金资助项目(Y2008D13)
作者单位
耿 越,赵 红 山东师范大学生命科学学院,山东省动物抗性生物学重点实验室,山东 济南 250014 
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中文摘要:
      目的 研究马尾松花粉多糖PPM60-A及其硫酸酯化物SPPM60-A对大鼠动脉平滑肌细胞 [Ca2+]i调控及增殖的影响。方法 常规水提醇沉法制备马尾松花粉多糖,Sephacryl S-400HR色谱分离得PPM60-A,氯磺酸-吡啶法得硫酸酯化物SPPM60-A,酯化度为1.28。酶解法分离制备大鼠动脉平滑肌细胞,测定酯化前后多糖对其胞内 [Ca2+]i和细胞增殖的影响。结果 PPM60-A和SPPM60-A均可以降低 [Ca2+]i,抑制高K+和去甲肾上腺素(NE)诱导的钙离子升高,降低高K+引起的钙离子水平上升,对NE诱导的血管主动脉平滑肌细胞增殖具有显著的抑制作用。PPM60-A作用效果好于SPPM60-A。结论 PPM60-A及SPPM60-A均能抑制细胞外Ca2+内流,抑制血管平滑肌细胞增殖。
英文摘要:
      Objective To explore the effects of polysaccharide PPM60-A from masson pine (Pinus massoniana) pollen and its sulfated derivative SPPM60-A on [Ca2+]i regulation and proliferation of arterial smooth muscle cells from rats. Methods Polysaccharide (PPM60-A) was extracted by hot water, classified by 60% ethanol from masson pine pollen and purified by Sephacryl S-400HR. Sulfated polysaccharide (SPPM60-A) was obtained from PPM60-A modified by chlorosulfonic acid-pyridin method and its esterification degree is 1.28. The original generation of vascular smooth muscle cells (VSMCs) was obtained by enzyme digestion with collagenae from artery of rats. The effects of PPM60-A and SPPM-60A on [Ca2+]i regulation and cell proliferation were determined. Results Both PPM60-A and SPPM60-A could decrease the [Ca2+]i significantly, inhibit the rising of calcium ion concentration induced by KCl and noradrenalin (NE); reduce the calcium ion level pretreated by KCl; inhibit the VSMCs proliferation induced by NE. Conclusion PPM60-A and SPPM60-A could inhibit the influx of calcium ion and the proliferation of VSMCs.
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