陆巍杰,唐永范,高瑞栋.HPLC法测定北冬虫夏草和常见食用菌中腺苷和虫草素[J].现代药物与临床,2011,26(4):313-315
HPLC法测定北冬虫夏草和常见食用菌中腺苷和虫草素
Determination of adenosine and cordycepin in Cordyceps militaris and common edible fungi by HPLC
  
DOI:10.7501/j.issn.1674-5515.[year].[issue].[sequence]
中文关键词:  北冬虫夏草  冬虫夏草  腺苷  虫草素  高效液相色谱
英文关键词:Cordyceps militaris (L.) Link.  Cordyceps sinensis (Berk.) Sacc.  adenosine  cordycepin  HPLC
基金项目:国家863计划重点项目(2007AA021502)
作者单位E-mail
陆巍杰 上海国宝企业发展中心上海 201203 geraldluweijie@hotmail.com 
唐永范   
高瑞栋 上海国宝生物工程研究所上海 201203  
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中文摘要:
      目的 建立一种HPLC法快速测定北冬虫夏草和几种常见食用菌中腺苷和虫草素的方法。方法 Welch Material XB-C18色谱柱(250 mm×4.6 mm, 5 μm),以水–甲醇(85︰15)为流动相,体积流量1.0 mL/min,检测波长258 nm,柱温40 ℃。结果 腺苷在0.023 2~0.464 0 μg、虫草素在0.030 0~0.600 0 μg与峰面积线性关系良好,平均加样回收率分别为98.97%、99.15%,RSD分别为1.41%、1.01%。结论 腺苷普遍存在于各种食用菌子实体中,而虫草素是虫草属真菌子实体中的特有活性成分。本法简便快速,为全面评估北冬虫夏草中核苷类成分的质量,推进人工培育北冬虫夏草替代野生冬虫夏草提供检测方法。
英文摘要:
      Objective To develop a rapid method by using HPLC to quantitatively determine adenosine and cordycepin in Cordyceps militaris products and common edible fungi. Methods The working conditions: column was Welch Material XB-C18 column (250 mm×4.6 mm, 5 μm), mobile phase was composed of water-methanol (85︰15) with flow rate of 1.0 mL/min and 258 nm detection wavelength, and temperature was 40 ℃. Results The linearity range of adenosine and cordycepin were 0.023 2—0.464 0 and 0.030—0.600 μg. The average recovery of adenosine and cordycepin were 98.97% and 99.15% with the RSD of 1.41% and 1.01%, respectively. Conclusion Adenosine appears widely in common edible fungi, and cordycepin is characteristic in fruitbodies of Cordyceps (Fr.) Link. The HPLC method is simple and fast., and could lay a method on the comprehensive evaluation of the nucleoside components in C. militaris, and promot the cultured C. militaris to replace the natural C. sinensis resources.
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