张富赓,张瑜,朱明丹,张少强,杜武勋,肖学凤.芪苈强心胶囊中人参皂苷类成分在大鼠体内药动学研究[J].现代药物与临床,2019,34(8):2268-2275
芪苈强心胶囊中人参皂苷类成分在大鼠体内药动学研究
Pharmacokinetics of ginsenosides in Qili Qiangxin Capsules in rat in vivo
投稿时间:2019-02-25  
DOI:10.7501/j.issn.1674-5515.2019.08.004
中文关键词:  芪苈强心胶囊  人参皂苷  药动学  HPLC-MS/MS
英文关键词:Qili Qiangxin Capsules  ginsenosides  pharmacokinetics  HPLC-MS/MS
基金项目:国家自然科学基金资助项目(81774227);天津市自然科学基金资助项目(17JCZDJC34600);天津市卫生和计划生育委员会中医中西医结合科研课题(2015050)
作者单位E-mail
张富赓 天津市环湖医院, 天津 300350
天津中医药大学, 天津 301617 
 
张瑜 天津中医药大学, 天津 301617  
朱明丹 天津中医药大学第二附属医院, 天津 300250  
张少强 天津中医药大学第二附属医院, 天津 300250  
杜武勋 天津中医药大学第二附属医院, 天津 300250 cnduwux@163.com 
肖学凤 天津中医药大学, 天津 301617 kai1219@163.com 
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中文摘要:
      目的 建立高效液相色谱–串联质谱(HPLC-MS/MS)法考察芪苈强心胶囊中人参皂苷类成分(人参皂苷Rb1、Rb2、Rg1、Rg3、Rc、Rd、Re、Rf、F2)在大鼠体内的药动学特征。方法 SD大鼠ig 1.3 g/kg芪苈强心胶囊混悬液,于给药后0.083、0.167、0.333、0.667、1、1.5、2、3、4、6、8、12、24 h经目内眦静脉丛取血,样品处理后,采用HPLC-MS/MS法测定血浆中人参皂苷Rb1、Rb2、Rg1、Rg3、Rc、Rd、Re、Rf、F2的血药浓度。通过DAS 3.0软件拟合计算药动学参数。结果 大鼠ig芪苈强心胶囊后,血浆中人参皂苷Rb2的药动学参数tmax为1.5 h,其余人参皂苷类成分的tmax均在0.67 h;AUC0-∞排序依次为人参皂苷Rb1 > 人参皂苷Rc > 人参皂苷Rg1 > 人参皂苷Re > 人参皂苷Rb2 > 人参皂苷Rd > 人参皂苷Rf > 人参皂苷Rg3 > 人参皂苷F2t1/2的排序依次为人参皂苷Rb2 > 人参皂苷Rb1 > 人参皂苷Rg1 > 人参皂苷Re > 人参皂苷F2 > 人参皂苷Rf > 人参皂苷Rd > 人参皂苷Rg3 > 人参皂苷Rc。结论 该检测方法专属性强,重复性好,可用于芪苈强心胶囊中人参皂苷类成分的药动学研究。
英文摘要:
      Objective To develop a high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method to study the pharmacokinetic characteristics of ginsenoside Rb1, Rb2, Rg1, Rg3, Rc, Rd, Re, Rf, and F2 in rat plasma after ig administration of Qili Qiangxin Capsules. Methods SD rats were given 1.3 g/kg Qili Qiangxin Capsules suspension by gavage. Blood samples were collected from inner canthus at 0.083, 0.167, 0.333, 0.667, 1, 1.5, 2, 3, 4, 6, 8, 12, and 24 h. Samples were treated, and the concentrations of ginsenoside Rb1, Rb2, Rg1, Rg3, Rc, Rd, Re, Rf, and F2 were determined by HPLC-MS/MS. The pharmacokinetic parameters were calculated by DAS 3.0 Software. Results The pharmacokinetic parameters tmax of ginsenoside Rb2 in rat plasma was 1.5 h, while 0.67 h for other ginsenosides. AUC0-∞ were in the order of ginsenoside Rb1 > Rc > Rg1> Re > Rb2 > Rd> Rf > Rg3 > F2. And t1/2 were in the order of ginsenoside Rb2 > Rb1 > Rg1 > Re > F2 > Rf > Rd > Rg3 > Rc. Conclusion The method is proved to be specific and repeatable, and to be suitable for determination of ginsenosides in Qili Qiangxin Capsules in rat plasma for pharmacokinetic study.
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