黄春兰,朱新华,叶慧文,李雄杰.HPLC法测定舒筋跌打膏中哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷和藁本内酯[J].现代药物与临床,2019,34(7):1960-1963
HPLC法测定舒筋跌打膏中哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷和藁本内酯
Determination of harpagide, harpagoside, catalpol, verbascoside, jionoside B1, martinoside, and ligustilide in Shujin Dieda Plaster by HPLC
投稿时间:2019-04-06  
DOI:10.7501/j.issn.1674-5515.2019.07.005
中文关键词:  舒筋跌打膏  哈巴苷  哈巴俄苷  梓醇  毛蕊花糖苷  焦地黄苯乙醇苷B1  马替诺皂苷  藁本内酯  高效液相色谱
英文关键词:Shujin Dieda Plaster  harpagide  harpagoside  catalpol  verbascoside  jionoside B1  martinoside  ligustilide  HPLC
基金项目:深圳市坪山区卫生系统科研项目(201705)
作者单位
黄春兰 深圳市坪山区人民医院 药剂科, 广东 深圳 518118 
朱新华 深圳市坪山区人民医院 药剂科, 广东 深圳 518118 
叶慧文 深圳市人民医院 药学部, 广东 深圳 518020 
李雄杰 武汉纺织大学 化学与化工学院, 湖北 武汉 430200 
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中文摘要:
      目的 建立HPLC波长切换法同时测定舒筋跌打膏中哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷、藁本内酯的方法。方法 Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5 μm);流动相:乙腈–0.2%磷酸溶液,梯度洗脱;0~31.0 min在210 nm波长下检测哈巴苷、哈巴俄苷和梓醇,31.0~55.0 min在330 nm波长下检测毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷和藁本内酯;体积流量0.9 mL/min;柱温30℃;进样量20 μL。结果 哈巴苷、哈巴俄苷、梓醇、毛蕊花糖苷、焦地黄苯乙醇苷B1、马替诺皂苷、藁本内酯分别在2.06~51.50、0.78~19.50、4.07~101.75、2.66~66.50、1.09~27.25、1.71~42.75、13.58~399.50μg/mL线性关系良好;各成分的平均回收率分别为99.41%、97.38%、99.18%、98.53%、97.98%、98.98%、99.96%,RSD值分别为0.86%、1.41%、1.11%、1.23%、0.92%、0.95%、0.76%。结论 该方法操作便捷、重复性好,可用于舒筋跌打膏的质量控制。
英文摘要:
      Objective To establish an HPLC with wavelength switching method for simultaneous determination of harpagide, harpagoside, catalpol, verbascoside, jionoside B1, martinoside, and ligustilide in Shujin Dieda Plaster. Methods The separation was performed on Agilent Eclipse XDB-C18 chromatographic column (250 mm×4.6 mm, 5 μm). The mobile phase was acetonitrile-0.2% phosphoric acid solution with gradient elution. The detection wavelengths were set at 210 nm for harpagide, harpagoside, and catalpol during 0-31.0 min, and verbascoside, jionoside B1, martinoside, and ligustilide were detected at 210 nm during 31.0-55.0 min. The flow rate was 0.9 mL/min, temperature of column was set at 30℃, and volume of injection was 20 ?L. Results The linear ranges of harpagide, harpagoside, catalpol, verbascoside, jionoside B1, martinoside, and ligustilide were 2.06-51.50 μg/mL, 0.78-19.50 μg/mL, 4.07-101.75 μg/mL, 2.66-66.50 μg/mL, 1.09-27.25 μg/mL, 1.71-42.75 μg/mL, 13.58-399.50 μg/mL, respectively. The average recoveries were 99.41%, 97.38%, 99.18%, 98.53%, 97.98%, 98.98%, and 99.96%, with RSD of 0.86%, 1.41%, 1.11%, 1.23%, 0.92%, 0.95%, and 0.76%, respectively. Conclusion The method is easy to operate and repeatable, Can be used for quality control of Shujin Dieda Plaster.
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