只德广,庞纪平,金兆祥,陈芙蓉,王玉晶,范迪,姜溪,岳南,孙双勇,顾煜琛.海马补肾丸对小鼠学习记忆障碍的改善作用[J].现代药物与临床,2019,34(5):1290-1293
海马补肾丸对小鼠学习记忆障碍的改善作用
Improvement of Haima Bushen Pills on learning and memory impairment in mice
投稿时间:2018-12-05  
DOI:10.7501/j.issn.1674-5515.2019.05.005
中文关键词:  海马补肾丸  学习记忆障碍  正确率  乙酰胆碱酯酶  超氧化物歧化酶  丙二醛
英文关键词:Haima Bushen Pills  learning and memory impairment  correct rate  acetylcholinesterase  SOD  MDA
基金项目:
作者单位E-mail
只德广 天津药物研究院新药评价有限公司, 天津 300301  
庞纪平 天津中新药业集团股份有限公司中新制药厂, 天津 300457
天津中新药业集团股份有限公司乐仁堂制药厂, 天津 300112 
 
金兆祥 天津中新药业集团股份有限公司乐仁堂制药厂, 天津 300112  
陈芙蓉 天津药物研究院新药评价有限公司, 天津 300301  
王玉晶 天津中新药业集团股份有限公司乐仁堂制药厂, 天津 300112  
范迪 天津中新药业集团股份有限公司乐仁堂制药厂, 天津 300112  
姜溪 天津药物研究院新药评价有限公司, 天津 300301  
岳南 天津药物研究院新药评价有限公司, 天津 300301  
孙双勇 天津药物研究院新药评价有限公司, 天津 300301  
顾煜琛 武警江苏省总队医院, 江苏扬州 225003 5001446@qq.com 
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中文摘要:
      目的 考察海马补肾丸对小鼠学习记忆障碍的改善作用,并探讨其作用机制。方法 小鼠随机分为对照组、模型组、吡拉西坦组、龟龄集组和海马补肾丸组。采用腹腔注射东莨菪碱制备小鼠记忆获得性障碍模型,计算学习正确率,测定蛋白含量和乙酰胆碱酯酶活力。采用腹腔注射亚硝酸钠制备小鼠记忆巩固性障碍模型,计算训练、测定学习正确率,测定蛋白含量、去甲肾上腺素(NE)、多巴胺(DA)、5-羟色胺(5-HT)以及丙二醛(MDA)、超氧化物歧化酶(SOD)含量。结果 海马补肾丸2.20 g/kg组能显著增加小鼠学习记忆的正确率(P<0.05),显著降低乙酰胆碱酯酶活力(P<0.01)。测定时,与模型组比较,海马补肾丸1.10、2.20 g/kg组能显著增加小鼠学习记忆正确率(P<0.05),能不同程度增加小鼠大脑SOD含量,降低MDA含量(P<0.05、0.01)。结论 海马补肾丸能明显改善小鼠的记忆获得、记忆巩固障碍,推测其作用机制主要是通过降低乙酰胆碱酯酶活性,增加脑内乙酰胆碱的含量,改善记忆获得能力;通过抗脑组织的氧化损伤,增加大脑SOD含量,降低MDA含量,改善记忆巩固能力。
英文摘要:
      Objective To investigate the improvement on Haima Bushen Pills on learning and memory impairment in mice, and study its mechanism. Methods Mice were randomly divided into control group, model group, piracetam group, Guilingji group, and Haima Bushen Pills group. The model of memory acquired impairment in mice was established by intraperitoneal injection of scopolamine. The correct rate of learning was calculated, and the protein content and acetylcholinesterase activity were determined. The memory consolidation impairment model of mice was induced by intraperitoneal injection of sodium nitrite. The learning accuracy of training and determination was calculated, and the protein content, NE, DA, 5-HT, MDA and SOD content were determined. Results Haima Bushen Pills (2.20 g/kg) group could significantly increase the correct rate of learning and memory in mice (P < 0.05), and significantly reduce the activity of acetylcholinesterase (P < 0.01). Compared with the model group, Haima Bushen Pills (1.10, 2.20 g/kg) group could significantly increase the correct rate of learning and memory in mice (P < 0.05), and increase SOD content and decrease MDA content in mice brain (P < 0.05, 0.01). Conclusion Haima Bushen Pills can significantly improve memory acquisition and memory consolidation disorders in mice. Its mechanism is maybe mainly through reducing the activity of acetylcholinesterase, increasing the content of acetylcholine in the brain and improving the ability of memory acquisition. And improve the memory consolidation by resisting oxidative damage of brain tissue, increasing SOD and reducing MDA content in brain.
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