陈桂荣,徐煜彬,冉小库,韩雪莹,窦德强.Compound 48/80诱导BN大鼠类过敏反应的蛋白组学研究[J].现代药物与临床,2019,34(5):1279-1284
Compound 48/80诱导BN大鼠类过敏反应的蛋白组学研究
Proteomic study of Compound 48/80 induced anaphylactoid reaction in BN rats
投稿时间:2018-09-20  
DOI:10.7501/j.issn.1674-5515.2019.05.003
中文关键词:  Compound 48/80  类过敏反应  蛋白组学  差异蛋白
英文关键词:Compound 48/80  anaphylactoid reaction  proteomics  differential protein
基金项目:国家自然科学基金青年科学基金资助项目(81303205);辽宁省高等学校创新人才计划项目(LR2017002);沈阳市中青年科技创新人才支持计划项目(RC170344)
作者单位E-mail
陈桂荣 辽宁中医药大学药学院, 辽宁 大连 116600  
徐煜彬 台州市中心医院台州学院附属医院, 浙江 台州 318000 gxr20080131@163.com 
冉小库 辽宁中医药大学药学院, 辽宁 大连 116600  
韩雪莹 辽宁中医药大学药学院, 辽宁 大连 116600  
窦德强 辽宁中医药大学药学院, 辽宁 大连 116600 deqiangdou@163.com 
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中文摘要:
      目的 探讨Compound 48/80诱导BN大鼠类过敏反应的发生机制,采用iTRAQ蛋白组学技术对类过敏的发生过程进行研究,并鉴定候选标志物。方法 将BN大鼠按体质量随机分为对照1组、2组和Compound 48/80 1组、2组,每组各6只。对照组iv生理盐水5 mL/kg,Compound 48/80组分别iv Compound 48/80 2.5 mL/kg,取血浆样品经蛋白提取、电泳和质谱分析,确定类过敏反应差异蛋白。结果 通过蛋白组学研究共鉴定10 585个肽段,总共鉴定1 987个蛋白质。提取7个与类过敏反应相关的差异蛋白。与对照组比较,Ica、Galectin-1上调;Hpse HEP、Mpo、MMP8、Hprt1、MMP9下调。结论 Hpse HEP、Mpo、MMP8、Hprt1、Ica、MMP9、Galectin-1均可以作为类过敏反应的候选生物标志物。
英文摘要:
      Objective To investigate the mechanism of Compound 48/80-induced anaphylactoid reaction in BN rats, and ITRAQ proteomics technique was used to study the occurrence of anaphylaxis, and identify candidate markers. Methods BN rats were randomly divided into control group 1 and 2, Compound 48/80 group 1 and 2 according to body weight, and each group had 6 rats. Rats in control group were iv administered with normal saline 5 mL/kg, and Compound 48/80 groups were iv Compound 48/80 2.5mL/kg. The plasma samples were analyzed by protein extraction, electrophoresis, and mass spectrometry to determine the differential proteins of anaphylactoid reaction. Results A total of 10 585 peptide segments and 1 987 proteins were identified by proteomics. Seven differential proteins related to anaphylactoid reaction were extracted. Compared with the control group, Ica and Galectin-1 were up-regulated, and Hpse HEP, Mpo, MMP8, Hprt1, and MMP9 were down-regulated. Conclusion Hpse HEP, Mpo, MMP8, Hprt1, Ica, MMP9, and Galectin-1 can be used as candidate biomarkers for anaphylaxis.
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