田会东,郭丽娜,贾明璐,陈雪平,王单单,王瑞.苍术–玄参药对治疗2型糖尿病作用机制的网络药理学研究[J].现代药物与临床,2019,34(5):1274-1278
苍术–玄参药对治疗2型糖尿病作用机制的网络药理学研究
Network pharmacology study on action mechanism of Atractylodis Rhizoma - Scrophulariae Rhizoma drug pair in treatment of type 2 diabetes mellitus
投稿时间:2018-12-26  
DOI:10.7501/j.issn.1674-5515.2019.05.002
中文关键词:  苍术  玄参  2型糖尿病  作用机制  网络药理学
英文关键词:network pharmacology  Atractylodis Rhizoma  Scrophulariae Rhizoma  type 2 diabetes mellitus  mechanism
基金项目:
作者单位E-mail
田会东 漯河市中心医院, 河南 漯河 462000  
郭丽娜 漯河市中心医院, 河南 漯河 462000  
贾明璐 漯河市中心医院, 河南 漯河 462000  
陈雪平 漯河市中心医院, 河南 漯河 462000  
王单单 漯河市中心医院, 河南 漯河 462000  
王瑞 漯河市中心医院, 河南 漯河 462000 yaoxuebu116@163.com 
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中文摘要:
      目的 采用网络药理学方法研究苍术–玄参药对治疗2型糖尿病的作用机制。方法 通过检索中药系统药理数据分析平台(TCMSP),把口服利用度(OB)≥20%,类药性(DL)≥0.1作为筛选条件,获得苍术、玄参主要活性成分和相关作用靶点。通过UniProt数据库提取作用靶点的基因名称,并用CTD网络在线分析平台获得与2型糖尿病相关的作用靶点,构建活性成分靶点相互作用的网络图和疾病靶点相互作用网络图。利用Cytoscape 3.6.1软件中的Merge功能进行网络合并,筛选交集网络,进一步进行GO功能和KEGG通路富集分析。结果 筛选得到27个活性分子,18个作用于2型糖尿病的靶点,KEGG富集通路37条,关键靶点包括胰岛素(INS)、一氧化氮合酶3(NOS3)、肿瘤坏死因子(TNF)等,关键通路包括长寿调节通路、胰岛素分泌、脂肪细胞因子信号通路、缺氧诱导因子-1(HIF-1)信号通路、视黄酸诱导基因蛋白I(RIG-I)样受体信号通路、晚期糖基化终末产物(AGE)及其受体(RAGE)的AGE-RAGE信号通路等。结论 苍术–玄参药对中的活性成分能通过多靶点、多通路起到治疗2型糖尿病的作用,为苍术–玄参药对的进一步开发提供了科学依据。
英文摘要:
      Objective To explore the action mechanism of Atractylodis Rhizoma - Scrophulariae Rhizoma drug pair in treatment of type 2 diabetes mellitus using network pharmacology. Methods All the chemical components related to Atractylodis Rhizoma - Scrophulariae Rhizoma from TCMSP database were searched. The oral bioavailability (OB) ≥ 20% and drug likeness (DL) ≥ 0.1 were used as the screening conditions for molecular compounds. The gene names of the targets were extracted from Uniprot database. The CTD analysis platform online was retrieved for the genes associated with type 2 diabetes mellitus, and then the component target PPI network and the disease target were mapped using the Cytoscape software. The Cytoscape 3.6.1 software was used to merge the network and filter the intersection network, and further analyze the gene GO function and the KEGG pathway enrichment. Results A total of 27 kinds of effective compounds, 18 target proteins, and 37 enrichment pathways were selected. Key target included INS, NOS3, and TNF etc. Regulation included longevity regulating pathway, insulin secretion, adipocytokine signaling pathway, HIF-1 signaling pathway, RIG-I signaling pathway, and AGE-RAGE signaling pathway in diabetic complications, etc. Conclusion Atractylodis Rhizoma - Scrophulariae Rhizoma drug pair and its active ingredients play therapeutic effect of type 2 diabetes mellitus through various target and multiple pathway, which provides the scientific basis for the development of Atractylodis Rhizoma - Scrophulariae Rhizoma drug pair.
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