SU Qi,SHANG Ping-ping,ZHANG Yong-min,JIA Na,HE Jiao,ZHAO Wen-na,SUN Wen-ji.HPLC Fingerprint and LC-TOF-MS Analysis on Extract from Roots of Gentiana macrophylla[J].Chinese Herbal Medicines (CHM),2012,4(3):245-251
HPLC Fingerprint and LC-TOF-MS Analysis on Extract from Roots of Gentiana macrophylla
Received:October 26, 2011  Revised:December 14, 2011
DOI:10.3969/j.issn.1674-6384.2012.03.012
中文关键词:  
英文关键词:fingerprint  Gentiana macrophylla  LC-ESI-TOF-MS  quality control  secoiridoid glucosides
基金项目:
Author NameAffiliation
SU Qi Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
SHANG Ping-ping Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
ZHANG Yong-min Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaNational Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China 
JIA Na Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
HE Jiao Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
ZHAO Wen-na Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
SUN Wen-ji Biomedicine Key Laboratory of Shaanxi Province, Northwest University, Xi’an 710069, China 
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中文摘要:
      
英文摘要:
      Objective Establishing a ?ngerprint method to identify the characteristic chemicals in the roots of Gentiana macrophylla and evaluate their quality. Methods RP-HPLC was developed for ?ngerprint analysis and determination of four ingredients in G. macrophylla roots from different sources. LC-ESI-TOF-MS was employed to identify the chromatographic peaks of the ?ngerprint. Results Five common peaks were identified by comparing their retention time with reference secoiridoid glucosides. Eight major peaks in chromatographic fingerprint were analyzed by on-line LC-ESI-TOF-MS. Four secoiridoid glucosides were identified based on their MS data. Conclusion The method is specific and could be served for the quality identi?cation and comprehensive evaluation of G. macrophylla.
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