WAN Jin-zhi,CHEN Xin-xia,QIU Chun-mei,LIN Xiang-cheng.Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography[J].Chinese Herbal Medicines (CHM),2010,2(2):148-152
Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography
Received:January 22, 2010  Revised:April 16, 2009
DOI:10.3969/j.issn.1674-6384.2010.02.010
中文关键词:  
英文关键词:Aloe vera L.  aloin  high-speed counter-current chromatography  isoaloeresin D  extraction and purification
基金项目:
Author NameAffiliation
WAN Jin-zhi Laboratory for Pharmaceutical Analysis and Quality Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China 
CHEN Xin-xia Laboratory for Pharmaceutical Analysis and Quality Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China 
QIU Chun-mei Laboratory for Pharmaceutical Analysis and Quality Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China 
LIN Xiang-cheng Institute of Fine Chemicals and Synthetic Drugs, Sun Yat-sen University, Guangzhou 510006, China 
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中文摘要:
      
英文摘要:
      Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production. Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A. vera. The biphasic solvent system composed of hexane-ethyl acetate-acetone-water (0.2︰5︰1.5︰5) was used at a flow rate of 1.0 mL/min, while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min. The effluent was detected at 254 nm. Results Isoaloeresin D (53.1 mg) and aloin (106.9 mg) were separated from the crude extract (384.7 mg) with the purities of 98.6% and 99.5%, respectively. Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.
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