Yu-fei Feng,Zhong-xu Jing,Yan-yan Zhang,Shao Lv,Qing-xia Guan,Zhi-xin Yang,Rui Wang,Yan-hong Wang.A guinea pig model of Ciwujia Injection-induced anaphylaxis for allergic substance screening[J].Chinese Herbal Medicines (CHM),2019,11(3):
A guinea pig model of Ciwujia Injection-induced anaphylaxis for allergic substance screening
  
DOI:10.1016/j.chmed.2019.03.006
中文关键词:  
英文关键词:anaphylaxis  Ciwujia Injection  guinea pig  model
基金项目:
Author NameAffiliation
Yu-fei Feng College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, China 
Zhong-xu Jing College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150041, China 
Yan-yan Zhang College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150042, China 
Shao Lv College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150043, China 
Qing-xia Guan College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150044, China 
Zhi-xin Yang College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150045, China 
Rui Wang College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150046, China 
Yan-hong Wang College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150047, China 
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中文摘要:
      
英文摘要:
      Objective: Though especially efficient for cardiovascular and cerebrovascular diseases treatment, many serious anaphylactic diseases could be induced by Ciwujia Injection (CWJI). However, study of the mechanism and detection of allergies have been investigated by the unknown sources of allergenic substances. In this study, a guinea pig model which could mimic the symptoms of anaphylactic reactions induced by Ciwujia Injection (CWJI) was modeled and used to screen the allergenic substance of CWJI. Methods: Guinea pigs were sensitized three times every other day with CWJI and excitated 14 d after the last sensitization administration. Then, the histamine, trypsin, IL-4 and IFN-γ levels, the Annexin V positive rate of peritoneal mast cells (PMC) were detected, the numbers of B lymphocyte and the pathological changes were also analyzed to verify the guinea pig allergy model, PCA test and IgE antibody levels were determined to study the mechanism. Results: The levels of total IgE, histamine, and trypsin were significantly increased after CWJI sensitization, IL-4 level was elevated, Annexin V positive of PMC cell rate, local skin reactions, and declined IFN-γ were observed after excitation. Histological examination showed that mild pathological changes in lungs were found. Conclusion: This guinea pig model may provide a powerful tool to study the mechanism in CWJI induced anaphylaxis and screen the allergic source of CWJI.
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