Xue-min Wei,Xiao-yue Wang,Zi-tong Gao,Pei Cao,Jian-ping Han.Identification of flower herbs in Chinese pharmacopoeia based on DNA barcoding[J].Chinese Herbal Medicines (CHM),2019,11(3):
Identification of flower herbs in Chinese pharmacopoeia based on DNA barcoding
  
DOI:10.1016/j.chmed.2019.05.003
中文关键词:  
英文关键词:adulteration  flower herbs  healthcare teas  ITS2  survey
基金项目:
Author NameAffiliation
Xue-min Wei Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China 
Xiao-yue Wang Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100194, China 
Zi-tong Gao Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100195, China 
Pei Cao Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100196, China 
Jian-ping Han Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100197, China 
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中文摘要:
      
英文摘要:
      Objective Flower herbs are an important category of traditional Chinese medicinal materials, some of which are used as healthcare tea in folk. However, the increasing adulteration of medicinal herbs is threatening consumer safety. The adulteration of flower herbs and their healthcare tea products in the market were investigated. Methods A total of 33 flower herb samples from several retail pharmacies in China were randomly collected and 27 flower healthcare tea samples were purchased online. They were identified using ITS2-based Traditional Chinese Medicine Database (TCMD). Additionally, Lonicerae Japonicae Flos and the adulterants were compared in the ITS2 secondary structures. Results There were one adulterant (Inulae Flos) in flower herb materials and eight adulterants in healthcare tea samples. Inula linariifolia was an adulterate species of Inulae Flos, Robinia pseudoacacia was of Sophorae Flos, and Lonicera macranthoides was of Lonicerae Japonicae Flos. Sophorae Flos and Lonicerae Japonicae Flos were two healthcare tea products with high adulteration rates. Conclusion The TCMD is powerful tool to identify flower herbs and the adulterants that frequently occurred in the flower herb market, especially online shops.
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