Nasim Javdan a,Seyed Abdulmajid Ayatollahi a,b,c*,Muhammad Iqbal Choudhary d,Safaa Al Hasani e,Farzad Kobarfard f,Kobra Mokhtarian g,Majid Khoshmirsafa h,Athar Ata b*.Tsc1/Tsc2 complex: A molecular target of capsaicin for protection against testicular torsion induced injury in rats[J].Chinese Herbal Medicines (CHM),2019,11(2):216-221
Tsc1/Tsc2 complex: A molecular target of capsaicin for protection against testicular torsion induced injury in rats
  
DOI:10.1016/j.chmed.2019.03.003
中文关键词:  
英文关键词:capsaicin  cell survival  molecular targets  testicular torsion  Tsc1/Tsc2 complex
基金项目:
Author NameAffiliation
Nasim Javdan a a Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran 
Seyed Abdulmajid Ayatollahi a,b,c* a Phytochemistry Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 19839-63113, Iran b Department of Chemistry, Richardson College for the Environmental Science Complex, The University of Winnipeg, Winnipeg, R3C 1X6, Canada. c Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences Tehran, 19839-63113, Iran 
Muhammad Iqbal Choudhary d d International Center for Chemical and Biological Sciences, H.E.J. Research Institute of Chemistry, University of Karachi, Karachi, 75270, Pakistan 
Safaa Al Hasani e e Reproductive Medicine Unit, University of Schleswig-Holstein, Luebeck, 24105, Germany 
Farzad Kobarfard f f Department of Medicinal Chemistry, Shaheed Beheshti School of Pharmacy, Tehran,19839-63113, Iran 
Kobra Mokhtarian g g Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord,1474717531, Iran 
Majid Khoshmirsafa h h Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran. 
Athar Ata b* b Department of Chemistry, Richardson College for the Environmental Science Complex, The University of Winnipeg, Winnipeg, R3C 1X6, Canada. 
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中文摘要:
      
英文摘要:
      Objective: The detailed knowledge about protective effects of capsaicin (cap) and involved mechanisms against testicular torsion (TT) is still not available completely. Methods: Male Wistar rats were assigned into four major cohorts: (i) sham, (ii) TT, (iii) three subgroups subjected to TT and different doses of cap (100, 500, and 1000 μg/mL), and (iv) three subgroups of healthy animals subjected to various concentrations of cap. The animals were decapitated at 24 h after reperfusion, and the evaluation of protein expression was performed by Western blotting assay. At 72 h after reperfusion, apoptotic cell death and tissue injury were evaluated by TUNEL nuclear and H&E staining, respectively. Results: The results showed that cap administration following TT significantly increased the expression of tuberous sclerosis proteins 1 and 2 (Tsc1/Tsc2) in a dose-dependent manner (P < 0.05). Cap decreased cell apoptosis at highest dose. Likewise, cap contributed to the preservation of tubular morphology and decreased tissue injury at the highest tested concentration (1000 μg/mL). Conclusion: Collectively, our findings demonstrate the validity of cap as a therapeutic agent against TT through targeting Tsc1/Tsc2 in a dose-dependent manner.
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