Xing-ying Zhai,Ling Zhang,Bing-tao Li,Yu-lin Feng,Guo-liang Xu,Hui Ouyang,Shi-lin Yang,Chen Jin *.Discrimination of toxic ingredient between raw and processed Pinellia ternata by UPLC/Q-TOF-MS/MS with principal component analysis and T-test[J].Chinese Herbal Medicines (CHM),2019,11(2):200-208
利用UPLC/Q-TOF-MS/MS主成分分析和T-test探讨生半夏与法半夏有毒成分的差异
Discrimination of toxic ingredient between raw and processed Pinellia ternata by UPLC/Q-TOF-MS/MS with principal component analysis and T-test
  
DOI:10.1016/j.chmed.2019.03.007
中文关键词:  差异  主成分分析  生半夏与法半夏  毒性成分  T-test  UPLC/Q-TOF-MS/MS
英文关键词:discrimination  principal component analysis  raw and processed Pinellia ternata (Thunb.) Berit.  toxic ingredient  T-test  UPLC/Q-TOF-MS/MS
基金项目:
Author NameAffiliation
Xing-ying Zhai Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Ling Zhang Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Bing-tao Li Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Yu-lin Feng Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Guo-liang Xu Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Hui Ouyang Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Shi-lin Yang Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
Chen Jin * Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China 
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中文摘要:
      目的:从化学成分探讨生半夏与法半夏的差异。方法:制备生半夏和法半夏样品并采用UPLC/Q-TOF-MS/MS测定,通过主成分分析(PCA)和T检验分析研究两组样品之间的差别,并确定相应的化学标记物。UPLC/Q-TOF-MS/MS可准确地测定得到荷质比和MS/MS 的碎片数据,通过参考文献或数据库对照相应数据我们可以鉴别相应化合物。结果:甘草苷、甘草素、溶血磷脂酰胆碱(LPC)是最具特征的化学标记物。由于LPC可导致炎症反应的发生,炮制前后LPC的减少是减毒的原因之一;甘草苷和甘草素有治疗炎症的作用且能减少肝损伤作用,其在炮制后的法半夏中的增加也是减毒的原因。结论:该方法不仅可探索传统中药炮制后减毒的机制还可分别对炮制前后中药的质量进行控制。
英文摘要:
      Objective: To investigate the toxicity difference between raw and processed Pinelliae Rhizoma (Banxia in Chinese, BX), the rhizoma of Pinellia ternata, from the view of chemical composition. Methods: Sixteen samples of raw and processed BX were prepared and analyzed by UPLC/Q-TOF-MS/MS. The discrimination (chemical markers) between the two group was investigated by principal component analysis (PCA) and T-test analysis. According to the accurate charge-to-mass ratio, MS/MS fragments, and comparision of corresponding data with the reference or database, the chemical markers were identified preliminarily. Results: Liquiritin, liquiritigenin, and lysophosphatidylcholine (LPC) were identified as the characteristic markers. The reducing of LPC in processed BX was one of the main reasons for detoxification because LPC could induce the inflammatory response; Liquiritin and liquiritigenin showed the anti-inflammatory effect and reduced liver injury, so the appearance of them in processed BX was an another reason for detoxification. Conclusion: An approach to explain the mechanisms of reducing the toxicity in medicinal plants by processing was proposed. Moreover, the chemical markers of toxicity could be used to differentiate the raw material from processed herbs for the quality control and safety application in clinical practice.
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