Md Iftekhar Ahmad,James E Keach,Tapan Behl,Pharkphoom Panichayupakaranant.Synergistic effect of α-mangostin on antibacterial activity of tetracycline, erythromycin, and clindamycin against acne involved bacteria[J].Chinese Herbal Medicines (CHM),2019,11(4):412-416
利用UPLC/Q-TOF-MS/MS主成分分析和T-test探讨生半夏与法半夏有毒成分的差异
Synergistic effect of α-mangostin on antibacterial activity of tetracycline, erythromycin, and clindamycin against acne involved bacteria
  
DOI:10.1016/j.chmed.2019.03.013
中文关键词:  差异  主成分分析  生半夏与法半夏  毒性成分  T-test  UPLC/Q-TOF-MS/MS
英文关键词:α-mangostin  acne  clindamycin  erythromycin  synergistic  tetracycline
基金项目:
Author NameAffiliation
Md Iftekhar Ahmad 1. Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand 
James E Keach 2. Department of Tropical Plant and Soil Sciences, University of Hawai‘i at Mānoa, Komohana Research and Extension Center, Hilo, Hawai‘i 96822, USA 
Tapan Behl 3. Department of Pharmacology, Chitkara College of Pharmacy, Chitkara University, Punjab 140401, India 
Pharkphoom Panichayupakaranant 1. Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand 4. Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai 90112, Thailand 
Hits: 105
Download times: 0
中文摘要:
      目的:从化学成分探讨生半夏与法半夏的差异。方法:制备生半夏和法半夏样品并采用UPLC/Q-TOF-MS/MS测定,通过主成分分析(PCA)和T检验分析研究两组样品之间的差别,并确定相应的化学标记物。UPLC/Q-TOF-MS/MS可准确地测定得到荷质比和MS/MS 的碎片数据,通过参考文献或数据库对照相应数据我们可以鉴别相应化合物。结果:甘草苷、甘草素、溶血磷脂酰胆碱(LPC)是最具特征的化学标记物。由于LPC可导致炎症反应的发生,炮制前后LPC的减少是减毒的原因之一;甘草苷和甘草素有治疗炎症的作用且能减少肝损伤作用,其在炮制后的法半夏中的增加也是减毒的原因。结论:该方法不仅可探索传统中药炮制后减毒的机制还可分别对炮制前后中药的质量进行控制。
英文摘要:
      Objective To evaluate the synergistic effect of α-mangostin with tetracycline, erythromycin, and clindamycin against bacteria involved in acne production. Methods A broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of α-mangostin and a range of antibiotics. Synergistic effects on antibacterial activity were determined based on their own MIC, and then by using a checkerboard method and a time-kill assay at 37 °C for 24 h. Results α-Mangostin exhibited antibacterial activity against Propionibacterium acnes, Staphylococcus aureus, S. epidermidis and S. pyogenes with MIC values of 0.78, 3.13, 0.78, and 6.25 μg/mL, respectively. The results of the checkerboard assay showed that α-mangostin produced synergistic effects with tetracycline, erythromycin and clindamycin against all tested bacteria, with a fractional inhibitory concentration index (FICI) between 0.09 and 0.32. Moreover, time-kill curve data indicated that α-mangostin increased the antibacterial activity of tetracycline, erythromycin and clindamycin. Conclusion These findings suggested that α-mangostin may be used to enhance the antibacterial activity of some antibiotics against bacteria involved in acne production.
HTML  View Full Text  View/Add Comment  Download reader
Close
Total Numbers of Visitors 1693369
CopyRight: Tianjin Press of Chinese Herbal Medicines
Address:308# An-shan West Road,Nankai District,Tianjin 300193,China
Tel:+86-22-23006901 Fax::+86-22-23006821 E_mail:chm@tiprpress.com
津备案:津ICP备13000267号 互联网药品信息服务资格证书编号:(津)-非经营性-2015-0031