Ming-li Zhu,Wei Wang,Feng Qin,Yong Chen*.Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats[J].Chinese Herbal Medicines (CHM),2019,11(2):177-184
加味脉君安片对自发性高血压大鼠血压的影响及其机制研究
Effects and mechanism of total phenols of Magnolia officinalis combined with Maijunan Tablets on blood pressure of spontaneous hypertensive rats
  
DOI:10.1016/j.chmed.2018.12.004
中文关键词:  厚朴总酚  脉君安片  内皮系统  氧化应激  肾素 - 血管紧张素 - 醛固酮  自发性高血压大鼠
英文关键词:endothelial function  Maijunan Tablets  oxidative stress  renin-angiotensin-aldosterone system  spontaneously hypertensive rats  total phenols of Magnolia officinalis
基金项目:
Author NameAffiliation
Ming-li Zhu Key Laboratory of Biotechnology of Hubei Province, Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University, Wuhan 430062, China 
Wei Wang Key Laboratory of Biotechnology of Hubei Province, Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University, Wuhan 430062, China 
Feng Qin Key Laboratory of Biotechnology of Hubei Province, Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University, Wuhan 430062, China 
Yong Chen* Key Laboratory of Biotechnology of Hubei Province, Key Laboratory of Biotechnology of Chinese Traditional Medicine, National & Local Joint Engineering Research Center of High-throughput Drug Screening Technology, Hubei University, Wuhan 430062, China 
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中文摘要:
      目的:薄膜包衣脉君安片由葛根,氢氯噻嗪,钩藤(366:1:980)及辅料混合成型,是我国中药保护品种。本次研究中使用的脉君安片(MJA)由葛根总黄酮,氢氯噻嗪,钩藤总碱(40:11:75)组成,加味脉君安片(M-MJA)是在脉君安片基础上加入厚朴总酚构成(葛根总黄酮:厚朴总酚:氢氯噻嗪:钩藤总碱=40:40:11:75)。本次研究主要是探讨M-MJA对自发性高血压大鼠(SHR)的降压作用和机制。 方法:将15只SHR随机分为对照组(MC)、脉君安片给药组(MJA,180 mg / kg?d)、加味脉君安片给药组(M-MJA,218 mg / kg?d),每组5只。每2周测血压 1次,8周后处死动物并检测与血压相关指标。 结果:结果表明M-MJA给药8周后收缩压与舒张压明显低于对照组。M-MJA可降低血浆中肾素,血管紧张素II,醛固酮的水平,降低内皮素-1和20-羟基二十碳四烯酸的水平,增加内皮性一氧化氮合酶水平,增加Nrf2的蛋白表达和血红素氧合酶-1,谷胱甘肽过氧化物酶的活性,抑制CYP2E1,CYP 4A的蛋白表达和丙二醛的活性。 结论:这些结果表明M-MJA可调节肾素 - 血管紧张素 - 醛固酮系统,改善内皮功能,抑制CYP4A活性,减少20-HETE的产生,缓解内脏器官的氧化应激损伤,最终产生抗高血压的作用。此外,M-MJA调节肾素 - 血管紧张素 - 醛固酮系统,改善内皮功能,抗氧化能力均比MJA更强,这表明M-MJA可能比MJA具有更好的抗高血压作用。
英文摘要:
      Objective: Maijunan (MJA) Tablets is a protected variety of traditional Chinese medicine (TCM) consisted of Pueraria lobata, hydrochlorothiazide (HTCZ), Uncaria rhynchophylla (366:1:980) and excipient. In the present work, MJA was consisted of the total flavones of P. lobata, HCTZ and total alkaloids of U. rhynchophylla (40:11:75). The combination of MJA and the total phenols of Magnolia officinalis (M-MJA) was consisted of the total flavones of P. lobata, the total phenols of M. officinalis, HCTZ and the total alkaloids of U. rhynchophylla (40:40:11:75). The aim of this work was to examine the effect and mechanism of M-MJA on the blood pressure of spontaneous hypertensive rats (SHRs). Methods: Adult male SHRs were randomly divided into control group, MJA group (180 mg/kg·d), and the M-MJA group (218 mg/kg·d) (n = 5). SHRs were orally administered with M-MJA and MJA respectively once a day for 8 weeks, the blood pressure of SHRs was measured every two weeks, and the biochemical indicators related to blood pressures were detected at the last dosing. Results: After oral administration of M-MJA to SHRs once a day for 8 weeks, the systolic and diastolic blood pressures of SHRs were deceased significantly. M-MJA affected renin-angiotensin-aldosterone system by decreasing the levels of Ren, Ang II and ALD, affected the endothelial function by decreasing the levels of ET-1 and 20-HETE, and increasing the level of eNOS, affected the oxidative stress by increasing the protein expression of Nrf2 and the activities of HO-1 and GSH-Px, and decreasing the protein expression of CYP2E1 and CYP4A, as well as the content of MDA. Conclusion: These results indicated that M-MJA could regulate the renin-angiotensin-aldosterone system, improve endothelial function, and inhibit CYP4A activity to reduce the production of 20-HETE, alleviate the oxidative stress disorder of the visceral organs, and eventually exert antihypertensive effect. Additionally, the anti-oxidant ability, regulating the renin-angiotensin-aldosterone system and improving endothelial function of M-MJA are more powerful than that of MJA, suggesting that M-MJA may have a better anti-hypertensive effect than MJA.
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