Shi-man Gao,Jiu-shi Liu,Min Wang,Ting-ting Cao,Yao-dong Qi,Ben-gang Zhang,Hai-tao Liu*,Xiao-bo Sun*,Pei-gen Xiao.Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods[J].Chinese Herbal Medicines (CHM),2019,11(2):160-168
基于定量和HPLC指纹图谱定性结合化学计量学分析的不同初加工方法党参药材的化学质量评价
Quantitative and HPLC fingerprint analysis combined with chemometrics for quality evaluation of Codonopsis Radix processed with different methods
  
DOI:10.1016/j.chmed.2018.08.012
中文关键词:  党参  指纹图谱  化学计量学  初加工方法  化学质量评价
英文关键词:Codonopsis Radix  fingerprint analysis  chemometrics  processing method  quality evaluation
基金项目:
Author NameAffiliation
Shi-man Gao Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Jiu-shi Liu Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Min Wang Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Ting-ting Cao Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Yao-dong Qi Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Ben-gang Zhang Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Hai-tao Liu* Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Xiao-bo Sun* Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
Pei-gen Xiao Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China 
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中文摘要:
      目的:研究不同初加工方法对党参药材化学质量的影响,为党参药材的产地初加工提供数据参考。 方法:定量和HPLC指纹图谱定性结合化学计量学的分析方法用于评价党参药材的化学质量。以甘肃省岷县党参药材规范化种植基地种植的党参药材为材料,通过HPLC指纹图谱的分析及党参炔苷、党参多糖、蔗糖、葡萄糖、果糖的含量测定,对烘干、晒干、阴干、硫熏、揉搓等不同初加工方法处理的党参药材样品进行综合的化学质量评价。 结果:不同初加工方法处理的党参药材样品的化学成分存在较大的差异。在党参药材的产地初加工过程中,建议采用晒干或阴干的初加工方法,避免采用硫熏的初加工方法。根据企业对党参药材规模化初加工的需求,可采用烘干的初加工方法,烘干温度建议采用40℃-50℃。另外,基于党参多糖、葡萄糖和果糖的含量,建议采用揉搓3次的初加工方法,基于党参炔苷和蔗糖的含量,应避免采用揉搓的初加工方法。 结论:该研究表明不同初加工方法对党参药材的化学质量具有较大的影响。研究结果针对不同的需求推荐了相应的初加工方法,为党参药材的产地初加工及后续的合理利用奠定了一定的基础。
英文摘要:
      Objective: To observe the effect of different processing methods on the quality of Codonopsis Radix and provide data reference for the reasonable processing in the producing areas. Methods: An analytical strategy that combined quantitative and HPLC ?ngerprint analysis with chemometrics was developed for the quality evaluation of Codonopsis Radix. Lobetyolin, polysaccharide, sucrose, glucose, and fructose were detected simultaneously in Codonopsis Radix samples treated with different processing methods including dryer-drying, sun-drying, shade-drying, sulfur fumigation and kneading, etc. Results: The results showed that different processing methods had an obvious impact on the quality of Codonopsis Radix. Sun-drying or shade-drying was recommended, and sulfur fumigation should be avoided. And dryer-drying at 40 °C ? 50 °C was suggested to meet the requirement for large scale processing. In addition, based on the content of polysaccharide, glucose, and fructose, it was advised to knead for three times. As for lobetyolin and sucrose, kneading should be avoided. Conclusions: Our ?ndings demonstrated that processing method had a big impact on the quality of Codonopsis Radix. Based on the results, the appropriate processing methods directed at different requirements were recommended. The study could lay a foundation for the reasonable processing of Codonopsis Radix in producing areas.
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